EXPRESSION OF SRC-LIKE ADAPTER PROTEIN MESSENGER-RNA IS INDUCED BY ALL-TRANS-RETINOIC ACID

By using a differential display method, specific bands were selected f rom ladder PCR products derived from ATRA-dependent differentiated U93 7 cells, in comparison with those of untreated U937, By screening the cDNA library of ATRA-dependent differentiated U937 cells with one of t he PCR products, we cloned the src-like adapter protein (SLAP), Northe rn blot analysis of U937 cells with or without ATRA treatment indicate d that the SLAP mRNA was clearly induced by ATRA, The induction was in hibited by the addition of cycloheximide, indicating that ATRA acted i ndirectly through synthesis of other proteins, The SLAP mRNA was induc ed in HL60 and NB-4 but not in K562 or THP-1. Interestingly, these cel ls in which SLAP mRNA was induced by ATRA all showed ATRA-dependent ce ll differentiation. The relationship between SLAP and cell differentia tion is unclear, but SLAP may transduce a signal for cell differentiat ion. (C) 1997 Academic Press