M. Daimon et al., IDENTIFICATION OF A HUMAN CDNA HOMOLOG TO THE DROSOPHILA TRANSLOCATION PROTEIN-1 (DTRP1), Biochemical and biophysical research communications, 230(1), 1997, pp. 100-104
In yeast, several integral membrane proteins such as Sec61p, Sec62p an
d Sec63p have been reported as the components involved in protein tran
slocation across and into the endoplasmic reticulum (ER) membrane. Amo
ng them, the homologues of Sec61p have been found both in bacterias an
d mammals, whereas those of Sec62p or Sec63p have not. So, Sec61p seem
to be the evolutionary conserved component, while Sec62p and Sec63p m
ay not. To date, no homologues of Sec62p have been found in mammals ye
t. Here, we report a novel human cDNA, HTP1 (for human translocation p
rotein 1), that encodes a protein of 399 amino acids that is 36.3% ide
ntical (64.6% similar) to Drosophila homologue of Sec62p, Drosophila t
ranslocation protein 1 (Dtrp1). Northern blot analysis showed two HTP1
transcripts of about 2.8 and 5.5 kb, which were expressed concomitant
ly in various human tissues such as heart, brain, placenta, liver and
pancreas. (C) 1997 Academic Press