NONRADIOACTIVE COLONY HYBRIDIZATION ASSAY FOR DETECTION AND ENUMERATION OF ENTEROLOXIGENIC CLOSTRIDIUM-PERFRINGENS IN RAW BEEF

Authors
BAEZ LA JUNEJA VK
Citation
La. Baez et Vk. Juneja, NONRADIOACTIVE COLONY HYBRIDIZATION ASSAY FOR DETECTION AND ENUMERATION OF ENTEROLOXIGENIC CLOSTRIDIUM-PERFRINGENS IN RAW BEEF, Applied and environmental microbiology, 61(2), 1995, pp. 807-810
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
2
Year of publication
1995
Pages
807 - 810
Database
ISI
SICI code
0099-2240(1995)61:2<807:NCHAFD>2.0.ZU;2-K
Abstract
A DNA probe endolabeled with digoxigenin by PCR was developed to detec t and enumerate enterotoxigenic Clostridium perfringens in raw beef. A fter 2 h of hybridization, membranes were developed by using an anti d igoxigenin-alkaline phosphatase conjugated antibody. The resulting chr omogenic reaction allowed us to detect and enumerate less than or equa l to 10 CFU of C. perfringens per g.