Dm. Morton et R. Tchao, REGULATION OF MOTILITY AND CYTOSKELETAL ORGANIZATION OF RAT BLADDER-CARCINOMA CELLS BY CYCLIC-AMP, Cell motility and the cytoskeleton, 29(4), 1994, pp. 375-382
Cyclic AMP (cAMP) has been implicated in the regulation of movement of
certain cultured cell types. We have studied the effects of cAMP on e
pithelial cell motility using serum-free NET-II cells, derived from a
rat bladder carcinoma. The random movement of these cells on type I co
llagen was reduced upon elevation of intracellular cAMP by several mea
ns and this effect was reversible. Alterations in the organization of
the cytoskeletal proteins F-actin and a-actinin occurred concurrently
with the reduction in motility, and the arrangement of these proteins
resembled that seen in non-motile cells on glass. In addition, pretrea
tment of cells with KT5720, a cAMP-dependent protein kinase (PKA)-spec
ific inhibitor, prevented the dibutyryl cAMP-induced reduction in cell
movement as well as the associated cytoskeletal changes. These result
s suggest that elevation of PKA is responsible for the observed effect
s on cell motility and cytoskeletal reorganization and demonstrate a r
ole for PKA in the regulation of cell motility in this system. (C) 199
4 Wiley-Liss, Inc.