GENOMIC DIVERSITY OF SMALL ROUND STRUCTURED VIRUSES IN THE UNITED-KINGDOM

Fifty-two faecal specimens collected in the United Kingdom between 198 6 and 1992, which contained small round structured virus (SRSV) partic les, were tested by reverse transcriptase polymerase chain reaction as says using two primer pairs derived from sequences of Snow Mountain Ag ent and Norwalk virus. There was poor correlation between results obta ined with each primer pair. Twenty specimens (38%) gave positive bands with SM51/31 primers and 18 (34%) were positive with SM52/32 primers, with a total of 30 specimens (57.7%) giving amplification products of the expected size with one or both primer pairs. Genomic variation wa s investigated by sequencing a 266 bp region of the RNA polymerase gen e from nine strains which had been antigenically typed by solid phase immune electron microscopy (SPIEM). RNA sequence identities ranged fro m 53 to 99%. Three genomic groups were suggested by phylogenic analysi s, the first of which contained Norwalk virus, Southampton virus, and strains typed by SPIEM as SRSV UK2. The second contained Snow Mountain agent and strains typed as either SRSV UK3 or UK4. The third containe d strains typed as SRSV UK1 and strains untypeable by SPIEM. Some corr elation was demonstrated when antigen typing by SPIEM and phylogenic g rouping based on sequence data were compared. (C) 1994 Wiley-Liss, Inc .