IN-VIVO DETECTION OF SUPEROXIDE ANION PRODUCTION BY THE BRAIN USING ACYTOCHROME-C ELECTRODE

A cytochrome c-coated platinized carbon electrode was utilized to dete ct superoxide generated by the brain during hypoxia/hypercarbia, focal ischemia, and reperfusion and following fluid percussion brain injury with and without hemorrhagic hypotension and reperfusion in the rat. All three of these forms of brain injury were associated with an incre ase in the superoxide signal. The cytochrome c electrode proved to be sensitive and responsive enough for minute-by-minute measurement of su peroxide generation by brain tissue.