DIRECT RESOLUTION OF ERGOT ALKALOID ENANTIOMERS ON A NOVEL CHIRAL SILICA-BASED STATIONARY-PHASE

A new covalently-bonded, silica-based stationary phase, using as the c hiral selector the 1-(3-aminopropyl) derivative of (+)-(5R,8S,10R)-ter guride, has been developed to resolve optically active isomers by HPLC . Good resolution of structurally related racemic ergot alkaloids were obtained using water-methanol mixtures as the eluent. Analysis of the influence of the type and concentration of the organic modifier, and the pH of the buffer in the mobile phase allowed the enantioseparation of these compounds to be optimized. Determination of the optical puri ty of a lisuride-containing drug is reported. (C) 1994 Wiley-Liss, Inc .