CHARACTERIZATION OF CDNAS ENCODING THE CHICK RETINOIC ACID RECEPTOR GAMMA-2 AND PREFERENTIAL DISTRIBUTION OF RETINOIC ACID RECEPTOR-GAMMA TRANSCRIPTS DURING CHICK SKIN DEVELOPMENT
Jj. Michaille et al., CHARACTERIZATION OF CDNAS ENCODING THE CHICK RETINOIC ACID RECEPTOR GAMMA-2 AND PREFERENTIAL DISTRIBUTION OF RETINOIC ACID RECEPTOR-GAMMA TRANSCRIPTS DURING CHICK SKIN DEVELOPMENT, Developmental dynamics, 201(4), 1994, pp. 334-343
Retinoic acid receptors alpha, beta and gamma (RAR alpha, beta and gam
ma) are ligand-inductible transcriptional activators which belong to t
he steroid/thyroid hormone receptor superfamily, At least two major is
oforms (I and 2) of each RAR arise by differential use of two promoter
s and alternative splicing. In mouse, the three RAR genes are expresse
d in stage- and tissue-specific patterns during embryonic development,
In order to understand the role of the different RARs in chick, RAR g
amma 2 cDNAs were isolated from an 8.5-day (stage 35 of Hamburger and
Hamilton) chick embryo skin library. The deduced chick RAR gamma S ami
no acid sequence displays uncommon features such as 21 specific amino
acid replacements, 12 of them being clustered in the amino-terminal re
gion (domains A2 and B), and a truncated acidic carboxy-terminal regio
n (F domain). However, the pattern of RAR gamma expression in chick em
bryo resembles that reported in mouse, particularly in skin where RAR
gamma expression occurs in both the dermal and epidermal layers at the
beginning of feather formation, and is subsequently restricted to the
differentiating epidermal cells, Northern blot analysis suggests that
different RAR gamma isoforms could be successively required during ch
ick development. (C) 1994 Wiley-Liss, Inc.