REARING WITH MONOCULAR LID SUTURE INDUCES ABNORMAL NADPH-DIAPHORASE STAINING IN THE LATERAL GENICULATE-NUCLEUS OF CATS

Citation
Ae. Gunluk et al., REARING WITH MONOCULAR LID SUTURE INDUCES ABNORMAL NADPH-DIAPHORASE STAINING IN THE LATERAL GENICULATE-NUCLEUS OF CATS, Journal of comparative neurology, 350(2), 1994, pp. 215-228
Citations number
47
Categorie Soggetti
Neurosciences
ISSN journal
00219967
Volume
350
Issue
2
Year of publication
1994
Pages
215 - 228
Database
ISI
SICI code
0021-9967(1994)350:2<215:RWMLSI>2.0.ZU;2-4
Abstract
We investigated the changes in NADPH-diaphorase staining that occur in the lateral geniculate nucleus of cats following rearing with monocul ar lid suture. This staining allows visualization of the synthesizing enzyme of nitric oxide, a neuromodulator associated with plasticity. I n the lateral geniculate nucleus of normally reared cats, NADPH-diapho rase exclusively labels the axons and terminals of an input from the p arabrachial region of the brainstem; no geniculate cells in the A-lami nae are labeled. Early monocular lid suture has no obvious effect on t he NADPH-diaphorase staining of parabrachial axons. However, this lid suture results in the abnormal appearance of NADPH-diaphorase staining for geniculate somata. These cells are located primarily in the nonde prived laminae. Double-labeling experiments indicate that these cells with abnormal NADPH-diaphorase reactivity are Y relay cells: NADPH-dia phorase staining is found in cells retrogradely labeled from visual co rtex; it is found in cells labeled with a monoclonal antibody for CAT- 301, which selectively targets Y cells; it is not found in cells label ed with an anti-GABA antibody, which targets interneurons. Also, NADPH -diaphorase labeled cells are among the largest cells in the nondepriv ed laminae, again suggesting that they are Y relay cells. We cannot su ggest a specific mechanism for this induction of NADPH-diaphorase labe ling, but it does not seem to be due to abnormal binocular competition induced by the monocular lid suture. (C) 1994 Wiley-Liss, Inc.