CD4-LYMPHOCYTE RATIO - EFFECTS OF REHYDRATION BEFORE EXERCISE IN DEHYDRATED MEN( CD8+ T)

Effects of fluid ingestion on CD4+/CD8+ T-lymphocyte cell ratios were measured in four dehydrated men (ages 30-46 yr) before and after 70 mi n of supine submaximal (71% VO2max) lower extremity cycle exercise. Ju st before exercise, Evans blue dye was injected for measurement of pla sma volume. The subjects then drank one of six fluid formulations (12 ml.kg(-1)) in 3-4 min. All six mean post-hydration (pre-exercise) CD4/CD8+ ratios (Becton-Dickinson Fluorescence Activated Cell Sorter and FACScan Consort-30 software program [San Jose, CA]) were below the nor mal range of 1.2-1.5; mean (+/- SE) and range were 0.77 +/- 0.12 and 0 .39-1.15, respectively. The post-exercise ratios increased: mean = 1.3 6 +/- 0.15 (P < 0.05) and range = 0.98-1.98. Regression of mean CD4+/C D8+ ratios on mean plasma osmolality resulted in pre- and post-exercis e correlation coefficients of -0.76 (P < 0.10) and -0.92 (P < 0.01), r espectively. The decreased pre-exercise ratios (after drinking) were p robably not caused by the Evans blue dye but appeared to be associated more with the stress (osmotic) of dehydration. The increased post-exe rcise ratios to normal levels accompanied the rehydration and were not due to the varied electrolyte and osmotic concentrations of the inges ted fluids or to the varied vascular volume shifts during exercise. Th us, the level of subject hydration and plasma osmolality may be factor s involved in the mechanism of immune system modulation induced by exe rcise.