D. Blanco et al., DETERMINATION OF FAT-SOLUBLE VITAMINS BY LIQUID-CHROMATOGRAPHY IN PEDIATRIC PARENTERAL NUTRITIONS, Journal of liquid chromatography, 17(20), 1994, pp. 4513-4530
A reversed phase high performance liquid chromatographic method using
narrow-bore columns packed with 3 mu m particles is described for the
simultaneous determination of transretinol, ergocalciferol, DL-alpha-t
ocopherol and phytomenadione. The fat-soluble vitamins were separated
in a C-18, bonded phase column and eluted with methanal as the eluent
pumped at a flow rate of 0.2 mL/min. A spectral detector was used and
the wavelenghts were set at 325, 265, 284 and 250 nm far vitamin A, D-
2, E and K-1 respectively. All vitamins were separated in less than 13
minutes This method was applied to the determination of fat-soluble v
itamins in the pediatric parenteral nutritions. The effect of the nutr
ition composition, daylight and the plastic infusion tubing on the sta
bility of the vitamins was studied. Recovery studies showed good resul
ts for all solutes (91.0% - 110.5%) and the coefficients of variation
were always less than 3%.