Jh. Ling et al., IDENTIFICATION OF THE IRON-CARBONYL STRETCH IN DISTAL HISTIDINE MUTANTS OF CARBONMONOXYMYOGLOBIN, Biochimica et biophysica acta. Bioenergetics, 1188(3), 1994, pp. 417-421
Soret-excitation resonance Raman (RR) spectra are reported for six dis
tal histidine mutants of carbonmonoxymyoglobin including H64A, H64V, H
64L, H64I, H64W, and H64W/L29F. Based on (CO)-C-13 isotope shifts, the
iron-carbonyl stretching vibrations are unambiguously identified. The
correct assignment of these modes eliminates the differences in the c
onformational substate occupations predicted by the RR versus IR data.