IDENTIFICATION OF THE IRON-CARBONYL STRETCH IN DISTAL HISTIDINE MUTANTS OF CARBONMONOXYMYOGLOBIN

Authors
LING JH LI TS OLSON JS BOCIAN DF
Citation
Jh. Ling et al., IDENTIFICATION OF THE IRON-CARBONYL STRETCH IN DISTAL HISTIDINE MUTANTS OF CARBONMONOXYMYOGLOBIN, Biochimica et biophysica acta. Bioenergetics, 1188(3), 1994, pp. 417-421
Citations number
25
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Bioenergetics → ACNP
ISSN journal
00052728
Volume
1188
Issue
3
Year of publication
1994
Pages
417 - 421
Database
ISI
SICI code
0005-2728(1994)1188:3<417:IOTISI>2.0.ZU;2-#
Abstract
Soret-excitation resonance Raman (RR) spectra are reported for six dis tal histidine mutants of carbonmonoxymyoglobin including H64A, H64V, H 64L, H64I, H64W, and H64W/L29F. Based on (CO)-C-13 isotope shifts, the iron-carbonyl stretching vibrations are unambiguously identified. The correct assignment of these modes eliminates the differences in the c onformational substate occupations predicted by the RR versus IR data.