ROLE OF ETHYLENE AND SHORT-CHAIN SATURATED FATTY-ACIDS IN THE SMOKE-STIMULATED GERMINATION OF CYCLOPIA SEED

The seeds of both Cyclopia intermedia and Cyclopia subternata exhibite d a seed coat imposed dormancy. However, seeds of the former species a lso exhibited an embryo dormancy which could be broken by exposure to smoke or ethylene. This stimulating effect of smoke and ethylene was i nhibited by exposure to NBD, indicating that ethylene in the smoke was responsible for the stimulation of germination. The involvement of et hylene in germination in both species was clearly demonstrated by the inhibition of germination after treatment with AOA. Differences in ger mination between the two species can not be ascribed to differences in ethylene production. Seeds of C. subternata, however, showed a much h igher capacity to bind ethylene than seeds of C. intermedia. Pulse tre atments with octanoic acid or smoke-saturated water stimulated germina tion in C. intermedia due to an increase in ethylene sensitivity, whil e longer treatments inhibited germination in both species. It appears that octanoic acid present in smoke-saturated water was responsible fo r the reaction of the seeds to the water.