IMMUNE-RESPONSE AND PROTEINS IN CEREBROSPINAL-FLUID AND AQUEOUS-HUMOR

The study was conducted on 372 subjects, i.e. 183 CFS samples and 189 aqueous humor samples with matched sera. Twelve different formulas by which the transferred and intrathecally/intraocularly synthesised frac tions of IgG and IgE can be analysed in CSF and aqueous humor, were te sted in control subjects and in pathological conditions. The controls were subjects with excluded nervous system disease or with senile cata racts. Pathologic situations were cataracts complicated by previous oc ular diseases (e.g inflammations, multiple sclerosis, etc.). The behav iour of oligoclonal IgG, IL-4 and beta 2-microglobulin levels in aqueo us humor, CFS and serum was observed. Based on results obtained, the a uthors suggested that measurement of beta 2-microglobulin and cytokine s (e.g IL4) in body fluids might represent useful adjunct to the detec tion of oligoclonal IgG and numerical evaluation of IgG or IgE synthes is in situations when intrathecal / intraocular immune response is obs erved by means of CFS and / or aqueous humor analysis. The differences of protein CFS samples are discussed in the context of the diagnostic procedures and application of protein transfer models.