Sl. Starcevic et Bs. Zielinski, IMMUNOHISTOCHEMICAL LOCALIZATION OF GLUTATHIONE-S-TRANSFERASE-PI IN RAINBOW-TROUT OLFACTORY RECEPTOR NEURONS, Neuroscience letters, 183(3), 1995, pp. 175-178
In the fish olfactory system, glutathione S-transferases (GST) which d
etoxify electrophilic substances and participate in reactions of lipop
hilic compounds, may be active in the biotransformation of odorants an
d xenobiotics. In this study GST activity in the rainbow trout olfacto
ry mucosa was high (477.6 +/- 218 nmol/min per mg protein). The GST pi
class was demonstrated by Western immunoblot analysis and localized b
y immunofluorescence to the dendritic and perinuclear regions of olfac
tory receptor neurons; areas previously shown to contain elevated glut
athione. The presence of GST and glutathione in fish olfactory recepto
r neurons suggests that these cells utilize the glutathione pathway.