Js. Lee et al., GENOMIC STRUCTURE OF C-KI-RAS PROTOONCOGENE OF THE HERMAPHRODITIC FISH RIVULUS-MARMORATUS (TELEOSTEI, RIVULIDAE), Biochemistry and molecular biology international, 35(1), 1995, pp. 57-63
The ras homologue of the rivulid fish Rivulus marmoratus was isolated
and characterized by screening about 3.0 x 10(5) genomic clones from a
Rivulus genomic library using human c-Ha-ras probe. When this clone w
as partially sequenced focusing on the region coding exons, it showed
97.5% amino acid homology to the human c-Ki-ras gene. The Rivulus c-Ki
-ras gene spans about 6.3 kb and consists of five exons including the
alternative splicing exon 4a/4b. The exon-intron boundaries of Rivulus
c-Ki-ras gene coincided with the GT/AG rule of consensus splice accep
tor and donor sequences as in mammalian c-Ki-ras genes. Amino acid seq
uence analysis of some domain regions of the Rivulus c-Ki-ras gene rev
ealed 100% identity to mammalian c-Ki-ras gene. This report is the fir
st that elucidate the entire structure of c-Ki-ras in a fish.