D. Galaris et P. Korantzopoulos, ON THE MOLECULAR MECHANISM OF METMYOGLOBIN-CATALYZED REDUCTION OF HYDROGEN-PEROXIDE BY ASCORBATE, Free radical biology & medicine, 22(4), 1997, pp. 657-667
Hydrogen peroxide induces rapid oxidation of metmyoglobin with an appa
rent second order rate constant, k(1) = 3.4 x 10(4) M(-1) min(-1). The
product of this interaction is ferrylmyoglobin with an unstable free
radical on the globin moiety. This activated form of myoglobin is able
: (a) to initiate the peroxidation of erythrocyte membranes and (b) to
form intra- and intermolecular covalent crosslinkings. The presence o
f ascorbic acid in amounts stoichiometric to H2O2 efficiently prevents
all the above processes. Moreover, in the presence of ascorbic acid a
cyclic process is taking place leading to H2O2 reduction, ascorbic ac
id oxidation, and unmodified metmyoglobin formation (reaction 1). asco
rbic acid; H2O2 -->(Mb) dehydroascorbate + 2H(2)O (1) The whole proces
s follows pseudofirst-order kinetics with the initial rate of ascorbic
acid oxidation dependent only on the concentration of H2O2 and indepe
ndent of ascorbic acid concentrations as low as the Limits of detectio
n. In order to explain the observed kinetics, it is proposed that asco
rbic acid is not directly involved in the reduction of ferryl- to metm
yoglobin, but it interacts, first with an intermediate in metmyoglobin
/H2O2 oxidation process, forming ferrylmyoglobin without the associate
d globin-centered free radical, and second, with a minor form of myogl
obin, with which ferrylmyoglobin is in equilibrium, forming, again, me
tmyoglobin. The rate-limiting step for the whole process seems to be t
he conversion of ferrylmyoglobin to this proposed minor form. These fi
ndings lead to the view that metmyoglobin, in the presence of ascorbat
e, may act like a pseudoperoxidase in conditions of oxidative stress i
n muscle, substituting for the low levels of catalase in this tissue.
Copyright (C) 1997 Elsevier Science Inc.