MOLECULAR-CLONING OF THE HUMAN GENE SUVCC1 ASSOCIATED WITH THE REPAIROF NONDIMER DNA-DAMAGE INDUCED BY SOLAR UV-RADIATION

A mutant cell line, DRP 287, sensitive to solar UV radiation and defic ient in the repair of solar UV-induced nondimer DNA damage, was derive d from ICR 2A frog cells. These cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensiti vity was restored and the repair defect corrected. The DNA from this s econdary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capa ble of restoring normal solar UV sensitivity and correcting the repair defect in the DRP 287 cells. This represents the first human gene whi ch has been isolated that is specifically involved in the repair of no ndimer DNA damage induced by solar UV radiation. It has been designate d SUVCC1 to denote solar UV cross-complementing gene number 1.