Morphological and immunological methods have revealed free-living Treb
ouxia cells among the first settlers in an area that has been complete
ly sterilized by a forest fire. These cells could be detected three ye
ars after the fire, and before any Lichen colony had been established.
We provide evidence that these free-living Trebouxia cells are identi
cal by morphological and immunological criteria with the photobiont of
Xanthoria parietina and of Buellia sp. that developed in another area
of the same region which was exposed to a forest fire four years earl
ier. It appeared that the X. parietina colony examined contained two (
or more) different Trebouxias.