Je. Nichols et al., EFFECTS OF CONDITIONED MEDIUM FROM DIFFERENT CULTURED-CELL TYPES AN AROMATASE EXPRESSION IN ADIPOSE STROMAL CELLS, Journal of the Society for Gynecologic Investigation, 2(1), 1995, pp. 45-50
OBJECTIVE: To determine whether serum-free (SF) conditioned media (CM)
from several human breast cancer cell lines and primary stromal cell
cultures contain factor(s) that mimic the marked stimulatory effects o
f serum on aromatase activity and aromatase P450 (P450arom) gene expre
ssion in adipose stromal cells in culture (ASC) in the presence of dex
amethasone (DEX). METHODS: Adipose stromal cells, harvested from fresh
adipose specimens, were grown to confluence, switched to SF media, an
d then incubated in the presence or absence of DEX with CM from T47-D
breast cancer cells, pre-treated with or without 17 beta-estradiol (E2
), and with CM from stromal cell cultures. Aromatase activity of the A
SC was determined by the [H-3]water release assay. Total RNA was isola
ted, and reverse transcription-polymerase chain reaction was performed
to determine the expression of various 5'-termini. RESULTS: T47-D CM
stimulated aromatase activity in a concentration-dependent manner, sim
ilar to that of serum, in ASC incubated with DEX. Estrogen potentiated
this in a dose-dependent fashion. The ASC CM and endometrial stromal
cell CM also markedly induced aromatase activity in ASC. Heat inactiva
tion destroyed the stimulating ability of CM. The majority of P450arom
5'-termini expressed by ASC incubated with CM plus DEX contained the
promoter 1.4-specific sequence. CONCLUSIONS: Conditioned media from se
veral breast cancer cell lines and primary stromal cell cultures can m
imic the effects of serum in the presence of DEX to stimulate aromatas
e activity in ASC. These results suggest that undefined, heat-labile a
nd proteinaceous factors are present in CM that stimulate P450arom exp
ression in a fashion similar to that of serum.