John Dalton described his own color blindness in 1794. In common with
his brother, he confused scarlet with green and pink with blue. Dalton
supposed that his vitreous humor was tinted blue, selectively absorbi
ng longer wavelengths. He instructed that his eyes should be examined
after his death, but the examination revealed that the humors were per
fectly clear. In experiments presented here, DNA extracted from his pr
eserved eye tissue showed that Dalton was a deuteranope, lacking the m
iddlewave photopigment of the retina. This diagnosis is shown to be co
mpatible with the historical record of his phenotype, although it cont
radicts Thomas Young's belief that Dalton was a protanope.