PULSE RADIOLYTIC STUDY OF THE OXIDATION REACTIONS OF URIC-ACID IN PRESENCE OF BOVINE SERUM-ALBUMIN - EVIDENCE OF POSSIBLE COMPLEX-FORMATIONIN THE TRANSIENT STATE
S. Adhikari et al., PULSE RADIOLYTIC STUDY OF THE OXIDATION REACTIONS OF URIC-ACID IN PRESENCE OF BOVINE SERUM-ALBUMIN - EVIDENCE OF POSSIBLE COMPLEX-FORMATIONIN THE TRANSIENT STATE, Radiation physics and chemistry, 49(1), 1997, pp. 47-50
Citations number
20
Categorie Soggetti
Nuclear Sciences & Tecnology","Chemistry Physical","Physics, Atomic, Molecular & Chemical
The pulse radiolytic and spectrophotometric study of uric acid in pres
ence of bovine serum albumin (BSA) has been carried out. In the spectr
ophotometric study there is no evidence for ground state interaction b
etween BSA and uric acid. The oxidation reactions of uric acid in pres
ence and absence of BSA employing CCl3OO. and Br-. radicals have been
carried out. In a composition of equal concentration of uric acid and
BSA, the CCl3OO. and Br-. radicals produce a transient absorption spec
trum which show two peaks at 330 and 360 nm. The peak at 360 nm is asc
ribed due to weak complex formation between semioxidised BSA and uric
acid radicals. The rate constant of CCl3OO. radical with uric acid inc
reases with the increase in BSA concentration which is explained as pr
otection of BSA by uric acid from radical attack. The Br-. radical att
acks uric acid and BSA in a manner similar to CCl3OO. radical. The bim
olecular rate constants for the reaction of Br radical with BSA and ur
ic acid have been found as 2.9 x 10(10) dm(3) mol(-1) s(-1) and 6.33 x
10(9) dm(3) mol(-1) s(-1), respectively. Copyright (C) 1997 Elsevier
Science Ltd