RADIOPROTECTIVE EFFECTS OF PROSTAGLANDINS FOR CHROMOSOMAL-ABERRATIONSAND CELL-KILLING IN V79 CHINESE-HAMSTER CELLS GROWN AS SPHEROIDS IN-VITRO AND FOR MOUSE SPERMATOGONIAL STEM-CELLS AND BONE-MARROW CELLS IN-VIVO

The radioprotective effects of prostaglandins (PGE(2), PGE(1) and its analogue misoprostol (MTP) were investigated in cultures of V79 Chines e hamster (CHO) cells grown as spheroids and as monolayers, CHO cells grown as monolayers, and in bone marrow polychromatic erythrocytes and spermatogonial stem cells in mouse. The X-ray doses were 0.75 Gy (ham ster cells) and 5, 8 and 10 Gy (mouse experiments). Prostaglandin pre- irradiation treatment resulted in a marked reduction in the frequencie s of chromosomal aberrations in V79 spheroids and of reciprocal transl ocations in mouse stem cell spermatogonia. The amount of mouse spermat ogonial stem cell killing was likewise significantly reduced. No radio protective effects ofprostaglandins could be demonstrated, however, fo r chromosomal aberrations in hamster cells grown as monolayers, for su rvival of V79 cells grown as spheroids, and for the induction of micro nuclei in bone marrow polychromatic erythrocytes of mouse.