Many species of birds are monomorphic and immatures never show seconda
ry sexual characters. The sex must be known in breeding programs or in
sex ratio studies. We were able to obtain chromosomes by lymphocyte c
ultures for ten species of birds. The slides were stained with Giemsa
or by use of the C-banding method which demonstrates heterochromatin.
Females are heterogametic (WZ) and males homogametic (ZZ). The sex of
these birds was determined either by a karyotype analysis (one pair of
chromosomes in the female being heterologous) or by identifying the W
chromosome (which is entirely heterochromatic) using C-banding. In so
me cases, the sex chromosomes could be recognised in metaphase cells w
ithout karyotyping.