O. Saiki et al., SIGNALING THROUGH CD40 RESCUES IGE BUT NOT IGG OR IGA SECRETION IN X-LINKED IMMUNODEFICIENCY WITH HYPER-IGM, The Journal of clinical investigation, 95(2), 1995, pp. 510-514
The ligand for CD40 (CD40L) is a membrane protein on activated T cells
that induces B cell proliferation and differentiation. Several mutati
ons of the CD40L gene were reported responsible for defective class sw
itching of B cells in an X-linked immunodeficiency with hyper IgM (X-H
IM). We studied four affected males from three families and found thre
e independent mutations including new mutations of CD40L gene. In ever
y X-HIM patient tested, however, anti-CD40 plus IL-10 did not induce c
lass switching from IgM to IgG or IgA, even in the presence of Staphyl
ococcus aureus Cowan I strain (SAC). CD4+ T cell clones, expressing CD
40L, on their surface, also did not rescue IgG or IgA induction by X-H
IM peripheral blood B cells in vitro. But signaling through CD40 induc
ed both B cell proliferation and IgE secretion when IL-4 was added to
the culture. Taken together, these results show that in vitro signalin
g through CD40 rescues IgE but not IgG or IgA secretion by peripheral
blood X-HIM B cells and suggest that in vivo CD40 and CD40L interactio
n might be necessary for IgG and IgA differentiation in X-HIM.