DETERMINATION OF CHOLESTEROL OXIDATION-PRODUCTS IN HUMAN PLASMA BY ISOTOPE-DILUTION MASS-SPECTROMETRY

A method based on isotope dilution-mass spectrometry was developed for the determination of nine cholesterol oxidation products in human pla sma. The cholesterol oxidation products determined were cholest-5-ene- 3 beta,7 alpha-diol, cholest-5-ene-3 beta,7 beta diol (7(alpha- and 7 beta-hydroxycholesterol, respectively), 3 beta-hydroxycholest-5-en-7-o ne (7-oxocholesterol),5,6 alpha-epoxy-5 alpha-cholestan-3 beta-ol (cho lesterol-5 alpha,6 alpha-epoxide), 5,6 beta-epoxy- 5 beta- cholestan-3 beta- ol (cholesterol-5 beta,6 beta- epoxide), cholestane-3 beta,5 al pha,6 beta-triol, cholest-5-ene-3 beta,24-diol (24-hydroxycholesterol) , cholest-5-ene-3 beta,25-diol (25-hydroxycholesterol), and cholest-5- ene-3 beta,27-diol (27-hydroxycholesterol). A corresponding deuterium- labeled internal standard, containing 3 to 6 deuterium atoms, was synt hesized for each cholesterol oxidation product except 5 beta,6 beta-ep oxycholesterol which was determined using the internal standard for 5 alpha,6 beta-epoxycholesterol. Plasma from 31 healthy volunteers was a nalyzed by the new method and 27-, 24-, and 7 beta-hydroxycholesterol were the most abundant cholesterol oxidation products (mean values 154 , 64, and 43 ng/ml, respectively). The other oxysterols determined wer e present in concentrations lower than 30 ng/ml. Males had higher 27-h ydroxycholesterol concentrations in plasma than females. The 5,6-oxyge nated products were present mainly unesterified while the other oxidat ion products were mostly in esterified form. (C) 1995 Academic Press, Inc.