One hundred eighty-eight accessions of Lycopersicon cheesmanii, L. chi
lense, L. chmielavskii, L. hirsutum, L. paviflorum, L. pennellii, and
L, peruvianum were screened for resistance to three isolates of tomato
spotted wilt virus (TSWV). All plants in an accession were initially
screened for resistance to TSWV using isolate 85-9 from Arkansas. Visu
al symptoms were used to cull obviously infected plants, followed by e
nzyme-linked immunosorbent assay (ELISA) to identify uninfected plants
. Cuttings were taken from uninfected plants in the first screening an
d the resulting plants were inoculated with isolates Glox and T-2 from
Texas and Hawaii, respectively. No resistance was identified in L. ch
eesmanii, L. chmielewskii, L, hirsutum, L, parviflorum, and L. pennell
ii. However, 33 of 63 L, chilense accessions produced 91 of 1268 plant
s that were uninfected with isolate 85-9 and 20 accessions that produc
ed 40 of 257 plants that were not infected with any of the isolates. A
fter screening with isolate 85-9 9 of 12 L. peruvianum accessions test
ed had 38 plants uninfected and 8 accessions had 25 plants that were n
ot infected with any of the isolates.