DNA-FINGERPRINTING IN SOYBEAN (GLYCINE-MAX (L) MERRILL) WITH OLIGONUCLEOTIDE PROBES FOR SIMPLE REPETITIVE SEQUENCES

Soybean DNA fingerprints were analyzed by digoxigenin-labeled oligonuc leotide probes complementary to simple repetitive sequences. The deare st and most polymorphic patterns were obtained with (AAT)(6) as a prob e, with which all 47 soybean cultivars tested could be distinguished. However, DNA fingerprints of individuals within cultivars showed the s ame pattern. Using (CT)(8), (GAA)(5) or (AAGG)(4) as probes, clear pol ymorphic patterns among cultivars and accessions in the subgenus Soja (Glycine max and Glycine soja) were not observed, while quite differen t patterns were found in accessions in the subgenus Glycine. The resul ts suggest that G. max and G. soja are closer in their genome structur e. DNA fingerprints of reciprocal crosses between cultivars and access ions in the subgenus Soja were similar, and contained bands of both pa rents. In an F-2 population from these crosses, such bands segregated in a Mendelian fashion.