The influence of the sulfate concentration and biofilm scraping on the
treatment performance and biofilm structure of aerobic fixed film rea
ctors was studied in rotating tubular reactors (RTRs). The RTRs operat
ed at 22(+/-3)degrees C treating diluted milk powder (0.9 g COD/1; COD
/SO42- ratio of 20) at an organic loading rate of 30 g COD per m(2) ca
rrier material per day. The reference RTR gave the following removal e
fficiencies: CODtot, 76%; CODsol, 91%; N-tot-N, 51%; N-org-N, 70%; PO4
3--P, 45%. COD removal was linear as a function of the reactor length
(2 mg COD/cm reactor). The biomass yield coefficient amounted to 0.44(
+/-0.13)g DM/g COD removed. High sulfate concentrations appreciably re
duced the yield to 0.28(+/-0.06)g DM/g CODrem but did not significantl
y influence the COD and nutrient removal capacity, despite the longer
hydraulic retention time (12'07'')compared to the reference RTR (6'25'
'). Daily removal of about 15% of the biofilm by biomass scraping did
not significantly affect the reactor performance. Biofilm samples grow
ing in the reference and the sulfate amended reactor showed comparable
specific oxygen uptake rates (15 mg O-2,/g VS.h). In the reactor trea
ting sulfate-rich wastewater, large populations (6.3-7.5 log CFU/g VS)
of lactate, acetate, propionate and butyrate oxidizing sulfate reduci
ng bacteria (SRB) were present. Their number as well as their sulfate
reducing activity (13.5 +/- 9.5 mg SO42-S/g VS.h) was higher compared
to the reference RTR. The addition of molybdate (30 mM) to the influen
t showed that SRB could account for 50% and 10% of the COD removal in
the sulfate amended and reference RTR, respectively. It is concluded t
hat wastewaters with a low COD/SO42- ratio are particularly suited for
aerobic fixed film processes because of the low waste biomass product
ion.