TRANSFERRIN POLYMORPHISM OF SOME RACES IN A LIVE GENE BANK OF COMMON CARP

Citation
C. Csizmadia et al., TRANSFERRIN POLYMORPHISM OF SOME RACES IN A LIVE GENE BANK OF COMMON CARP, Aquaculture, 129(1-4), 1995, pp. 193-198
Citations number
10
Categorie Soggetti
Fisheries,"Marine & Freshwater Biology
Journal title
ISSN journal
00448486
Volume
129
Issue
1-4
Year of publication
1995
Pages
193 - 198
Database
ISI
SICI code
0044-8486(1995)129:1-4<193:TPOSRI>2.0.ZU;2-Q
Abstract
Conservation of genetic variety of strains maintained in live gene ban ks is a high-priority task. Description of the genetic structure shoul d be the first step in this work. By applying different biochemical-ge netic markers such as transferrin and isoenzymes, the individuals and the populations could be well characterised genetically. Based on this , breeding programs as well as conservation of races can be carried ou t without disappearance of genes from the pool. The aim of this survey was to describe the polymorphism of transferrins of carp strains in t he live gene bank maintained at the Fish Culture Research Institute, S zarvas, Hungary. Biochemical polymorphism was studied by standard meth ods of horizontal electrophoresis in starch gel as well as vertical el ectrophoresis in polyacrylamid gel. A total of 840 breeders of 15 carp races were sampled. Identification of transferrin genotypes was made by the traditional classification. In the 15 carp populations, 20 tran sferrin genotypes were described (AA, BE, DD, EE, FF, GG, AB, AD, AF, AG, ED, BE, BG, DE, DF, DG, EF; EG, FG, and FH) in the presence of 7 a lleles (A, B, D, E, F, H and G). The frequency of allele D was very hi gh (0.34-0.62) in all populations except one (koi). It was remarkable that the frequency of allele D was very low (0.0698) in this group sho wing the highest variability, while the frequency of allele B, being v ery rare in all other races, was relatively high (0.27) in the group o f koi.