A new malate dehydrogenase (MDH) locus (Mdh) was identified in cucumbe
r (Cucumis sativus L.) by using an extraction buffer containing sucros
e and 2-mercaptoethanol. This fourth locus localizes on starch gels be
tween the previously described Mdh-2 and Mdh-3 loci, necessitating the
renaming of existing loci. Given previous MDH nomenclature in cucumbe
r, this locus should be Mdh-3, and the former Mdh-3 is more correctly
labeled Mdh-4. Mdh-x designates the interlocus heteromer formed betwee
n Mdh-2 and Mdh-4. Interpreting our data in terms of previous work, we
hypothesize that Mdh-1 codes for a microbody isozyme; Mdh-2, Mdh-4, a
nd Mdh-x code for cytosolic isozymes; and Mdh-3 codes for a mitochondr
ial isozyme.