A 4TH MALATE-DEHYDROGENASE (MDH) LOCUS IN CUCUMBER

Citation
Ld. Knerr et al., A 4TH MALATE-DEHYDROGENASE (MDH) LOCUS IN CUCUMBER, HortScience, 30(1), 1995, pp. 118-119
Citations number
7
Categorie Soggetti
Horticulture
Journal title
ISSN journal
00185345
Volume
30
Issue
1
Year of publication
1995
Pages
118 - 119
Database
ISI
SICI code
0018-5345(1995)30:1<118:A4M(LI>2.0.ZU;2-0
Abstract
A new malate dehydrogenase (MDH) locus (Mdh) was identified in cucumbe r (Cucumis sativus L.) by using an extraction buffer containing sucros e and 2-mercaptoethanol. This fourth locus localizes on starch gels be tween the previously described Mdh-2 and Mdh-3 loci, necessitating the renaming of existing loci. Given previous MDH nomenclature in cucumbe r, this locus should be Mdh-3, and the former Mdh-3 is more correctly labeled Mdh-4. Mdh-x designates the interlocus heteromer formed betwee n Mdh-2 and Mdh-4. Interpreting our data in terms of previous work, we hypothesize that Mdh-1 codes for a microbody isozyme; Mdh-2, Mdh-4, a nd Mdh-x code for cytosolic isozymes; and Mdh-3 codes for a mitochondr ial isozyme.