ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR IGA AND IGG ANTIBODIES TO EPSTEIN-BARR-VIRUS RIBONUCLEOTIDE REDUCTASE IN PATIENTS WITH NASOPHARYNGEAL CARCINOMA

Authors
FONESTAN A CHAN SH TSAO SY GAN LH TAN WH LI B KHONG PW GAN YY
Citation
A. Fonestan et al., ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR IGA AND IGG ANTIBODIES TO EPSTEIN-BARR-VIRUS RIBONUCLEOTIDE REDUCTASE IN PATIENTS WITH NASOPHARYNGEAL CARCINOMA, International journal of cancer, 59(6), 1994, pp. 739-742
Citations number
20
Categorie Soggetti
Oncology
Journal title
International journal of cancer → ACNP
ISSN journal
00207136
Volume
59
Issue
6
Year of publication
1994
Pages
739 - 742
Database
ISI
SICI code
0020-7136(1994)59:6<739:E(FIAI>2.0.ZU;2-0
Abstract
661 bp coding for the carboxyl end of the large sub-unit of EBV ribonu cleotide reductase was cloned into the pMal plasmid vector. Purified r ecombinant protein was tested in IgG and IgA ELISAs. For the IgG assay , 81 out of 100 NPC patients tested positive, whereas for the IgA assa y, 60 tested positive. Among 100 normal individuals, I tested positive for the IgG assay and 9 tested positive for the IgA assay. The IgG as say picked up 6 out of 19 NPC sera which were IFA-VCA- and IFA-EA-nega tive for IgA antibodies. Hence the recombinant ribonucleotide reductas e could have good potential as a diagnostic test for NPC or could serv e as a complementary test to IFA. (C) 1994 Wiley-Liss, Inc.