THE JELLYFISH GREEN FLUORESCENT PROTEIN - A NEW TOOL FOR STUDYING ION-CHANNEL EXPRESSION AND FUNCTION

Authors
MARSHALL J MOLLOY R MOSS GWJ HOWE JR HUGHES TE
Citation
J. Marshall et al., THE JELLYFISH GREEN FLUORESCENT PROTEIN - A NEW TOOL FOR STUDYING ION-CHANNEL EXPRESSION AND FUNCTION, Neuron, 14(2), 1995, pp. 211-215
Citations number
16
Categorie Soggetti
Neurosciences
Journal title
Neuron → ACNP
ISSN journal
08966273
Volume
14
Issue
2
Year of publication
1995
Pages
211 - 215
Database
ISI
SICI code
0896-6273(1995)14:2<211:TJGFP->2.0.ZU;2-U
Abstract
Two methods are described for using the jellyfish green fluorescent pr otein (GFB) as a reporter gene for ion channel expression. GFP fluores cence can be used to identify the transfected cells, and to estimate t he relative levels of ion channel expression, in cotransfection experi ments. A GFP-NMDAR1 chimera can be constructed that produces a functio nal, fluorescent receptor subunit. These methods should facilitate stu dies of ion channel expression, localization, and processing.