N. Senda et al., A HIGHLY SENSITIVE METHOD TO QUANTIFY TRIAZOLAM AND ITS METABOLITES WITH LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY, BMC. Biomedical chromatography, 9(1), 1995, pp. 48-51
We established a highly sensitive method to determine triazolam and it
s major metabolites, alpha-hydroxytriazolam and 4-hydroxytriazolam, in
human plasma and urine with a liquid chromatography-mass spectrometry
system which incorporates an atmospheric chemical ionization interfac
e. A plasma sample and a urine sample after solvent extraction were in
jected into an ODS column of reversed phase with a mobile phase in a l
inear solvent gradient of initially 50 mM ammonium acetate (pH 4.0) 50
%:methanol 50% and 15 min later methanol 100%; quantification limits o
f as low as 20 pg/mL at an SNR of 3 were obtained for each compound. W
ith diazepam as an internal standard, recovery yields of 84, 81, and 7
7% were obtained for triazolam, alpha-hydroxytriazolam and 4-hydroxytr
iazolam, respectively,