VIRAL Q-BETA RNA AS A HIGH EXPRESSION VECTOR FOR MESSENGER-RNA TRANSLATION IN A CELL-FREE SYSTEM

Authors
KATANAEV VL KURNASOV OV SPIRIN AS
Citation
Vl. Katanaev et al., VIRAL Q-BETA RNA AS A HIGH EXPRESSION VECTOR FOR MESSENGER-RNA TRANSLATION IN A CELL-FREE SYSTEM, FEBS letters, 359(1), 1995, pp. 89-92
Citations number
19
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
359
Issue
1
Year of publication
1995
Pages
89 - 92
Database
ISI
SICI code
0014-5793(1995)359:1<89:VQRAAH>2.0.ZU;2-A
Abstract
Dihydrofolate reductase (DHFR) mRNA was inserted into Q beta phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesi s of DHFR, which was two orders of magnitude higher than that in the c ase of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Q beta replicase as compared with its synthesis when the original Q beta RNA was used.