IN-VIVO EFFECTS OF HYPERGLYCEMIA ON THE OUTCOME OF ACUTE MESANGIAL INJURY IN RATS

To assess the effects of high blood glucose concentration on glomerula r changes after the acute mesangial cell injury in the rat, the monocl onal anti-Thy1.1 antibody OX-7 was injected into streptozoticin-induce d diabetic rats or normal rats. The increase in proliferating cell nuc lear antigen-positive cells in glomeruli at day 4 and glomerular hyper cellularity at day 18 was less prominent in diabetic rats than in norm al rats. The expansion of mesangial matrix area assessed by fibronecti n immunostaining was more prominent, and segmental glomerulosclerosis was observed at day 60 in the diabetic rats. These data suggest that t he insulin-deficient group may reflect impaired ''wound-healing,'' lea ding to the prolonged ECM accumulation under the hyperglycemic conditi on in vivo.