THE INTERACTIONS OF ANTI-MLI MONOCLONAL-ANTIBODIES WITH ISOFORMS OF THE LECTIN FROM VISCUM-ALBUM

Authors
TONEVITSKY AG RAKHMANOVA VA AGAPOV II SHAMSHIEV AT USACHEVA EA PROKOPHEV SA DENISENKO ON ALEKSEEV YO PFUELLER U
Citation
Ag. Tonevitsky et al., THE INTERACTIONS OF ANTI-MLI MONOCLONAL-ANTIBODIES WITH ISOFORMS OF THE LECTIN FROM VISCUM-ALBUM, Immunology letters, 44(1), 1995, pp. 31-34
Citations number
10
Categorie Soggetti
Immunology
Journal title
Immunology letters → ACNP
ISSN journal
01652478
Volume
44
Issue
1
Year of publication
1995
Pages
31 - 34
Database
ISI
SICI code
0165-2478(1995)44:1<31:TIOAMW>2.0.ZU;2-#
Abstract
Monoclonal antibodies (mAb) reacting with native (TA5, TB12) and denat ured (T33, T35) plant toxin mistletoe lectin I (MLI) from Viscum album have been obtained. The interaction between mAbs and native toxin wit h ML isoforms (MLII, MLIII) has been investigated. An immunological cr oss-reaction has been shown to take place for mAb TA5 (anti-A-chain of MLI) between MLII and MLIII isoforms of toxin. TA5 has not inhibited enzyme activity of the A-chain in a rabbit reticulocyte cell-free syst em. TB12 has been shown to react with the galactose-binding site of th e B-chain. TA5 and TB12 have shown no cross-reaction with plant toxin ricin. The association constants for mAbs have been determined. The na ture of heterogeneity of the lectins from Viscum album is discussed.