METHOD FOR RAPID REMOVAL OF ETHANOL FROM CHLOROFORM IN SOIL MICROBIALBIOMASS DETERMINATIONS

Authors
WILLIAMSON WM BLUNT JW GREENFIELD LG
Citation
Wm. Williamson et al., METHOD FOR RAPID REMOVAL OF ETHANOL FROM CHLOROFORM IN SOIL MICROBIALBIOMASS DETERMINATIONS, Communications in soil science and plant analysis, 26(3-4), 1995, pp. 407-410
Citations number
5
Categorie Soggetti
Agriculture Soil Science","Plant Sciences","Chemistry Analytical
Journal title
Communications in soil science and plant analysis → ACNP
ISSN journal
00103624
Volume
26
Issue
3-4
Year of publication
1995
Pages
407 - 410
Database
ISI
SICI code
0010-3624(1995)26:3-4<407:MFRROE>2.0.ZU;2-F
Abstract
A method is described for the removal of stabilising ethanol from reag ent-grade chloroform (CHCl3) which is quicker, safer, and as effective and reliable as the standard method described in Jenkinson and Powlso n (1976). The proposed method foregoes the use of concentrated acid an d distillation and involves shaking CHCl3 with distilled water followe d by drying the final chloroform phase over anhydrous calcium chloride (CaCl2). Both methods yielded CHCl3 containing residual ethanol of le ss than 0.001% w/w.