T. Royo et al., IMMUNOLOCALIZATION OF MITOCHONDRIAL 3-HYDROXY-3-METHYLGLUTARYL COA SYNTHASE IN RAT-LIVER, Journal of cellular physiology, 162(1), 1995, pp. 103-109
We report the preparation of specific polyclonal antibodies raised aga
inst two synthetic peptides deduced from the DNA sequence for the rat
liver mitochondrial 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) sy
nthase gene. Immunoelectron microscopy using these antibodies on hepat
ic cryoultrathin sections confirms the mitochondrial localization of t
his protein in hepatocytes. Immunofluorescence microscopy on frozen se
ctions of adult rat liver revealed fluorescence inside all hepatocytes
, with no evidence of zonation, indicating that ketogenesis may not be
limited to specific regions of rat liver but is extended to all hepat
ocytes. (C) 1995 Wiley-Liss, Inc.