ABSENCE OF P350 SUBUNIT OF DNA-ACTIVATED PROTEIN-KINASE FROM A RADIOSENSITIVE HUMAN CELL-LINE

Authors
LEESMILLER SP GODBOUT R CHAN DW WEINFELD M DAY RS BARRON GM ALLALUNISTURNER J
Citation
Sp. Leesmiller et al., ABSENCE OF P350 SUBUNIT OF DNA-ACTIVATED PROTEIN-KINASE FROM A RADIOSENSITIVE HUMAN CELL-LINE, Science, 267(5201), 1995, pp. 1183-1185
Citations number
54
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Science → ACNP
ISSN journal
00368075
Volume
267
Issue
5201
Year of publication
1995
Pages
1183 - 1185
Database
ISI
SICI code
0036-8075(1995)267:5201<1183:AOPSOD>2.0.ZU;2-K
Abstract
The radiosensitive rodent mutant cell line xrs-5 is defective in DNA d ouble-strand break repair and lacks the Ku component of the DNA-activa ted protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found t o be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.