COMPOSITE SORBENTS FOR LIQUID-CHROMATOGRAPHY - A SIZE-EXCLUSION STUDYOF DEXTRAN GEL INCORPORATED INTO POROUS SOLID PARTICLES

The novel class of column packings for high-performance liquid chromat ography was prepared. These materials are formed by silica-, polyhydro xyethylmethacrylate-, or carbon-based macroporous solid particles with pores filled by a soft dextran network. They were examined by inverse size-exclusion chromatography. The analytes, which are small enough t o penetrate the dextran network, can interact selectively with the sup port surface. Such interactions may be either suppressed by an appropr iate deactivation of the support surface prior to dextran incorporatio n or utilized for special applications in which retention of small mol ecules of analytes is combined with steric exclusion of large molecule s of interference.