Ae. Ivanov et al., CHARACTERIZATION OF WEAK HYDROPHOBIC COMPOSITE SORBENTS AND THEIR APPLICATION TO THE ISOLATION OF BACTERIAL LECTIN, Journal of chromatography B. Biomedical applications, 664(1), 1995, pp. 219-223
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
Composite sorbents based on wide-pore glass and silica coated with N-b
utyl polyacrylamide (butyl-PA-glass and butyl-PA-silica) were studied.
The surface tension of butyl-PA-silica is 50-55 mJ/m(2) as evaluated
by the sedimentation volume technique. The linear dependences of log k
' on ammonium sulphate concentration were determined by isocratic chro
matography of the dipetide Trp-Trp and lysozyme on butyl-PA-glass. Bot
h solutes were shown to have a weaker retention on butyl-PA-glass than
on butyl-Toyopearl 650C. This weaker retention is beneficial in the p
urification of sialic acid-binding lectin from Bacillus subtilis.