CHARACTERIZATION OF WEAK HYDROPHOBIC COMPOSITE SORBENTS AND THEIR APPLICATION TO THE ISOLATION OF BACTERIAL LECTIN

Composite sorbents based on wide-pore glass and silica coated with N-b utyl polyacrylamide (butyl-PA-glass and butyl-PA-silica) were studied. The surface tension of butyl-PA-silica is 50-55 mJ/m(2) as evaluated by the sedimentation volume technique. The linear dependences of log k ' on ammonium sulphate concentration were determined by isocratic chro matography of the dipetide Trp-Trp and lysozyme on butyl-PA-glass. Bot h solutes were shown to have a weaker retention on butyl-PA-glass than on butyl-Toyopearl 650C. This weaker retention is beneficial in the p urification of sialic acid-binding lectin from Bacillus subtilis.