Permeable large-pore particles have many applications, in particular i
n perfusion chromatography for bioseparations. The objective of this p
aper is to elucidate the mass transport mechanisms in two commercial a
dsorbents-POROS Q/M and Q Hyper D-and to answer the question if intrap
article convection is present as a mass transfer mechanism. The paper
contains three sections. In the first part, mass transfer inside porou
s particles is discussed. The mass transfer mechanism which allows imp
roved performance of perfusion chromatography is intraparticle convect
ion. The combined effect of intraparticle convection and diffusion is
an ''augmented'' effective diffusivity. This is the key concept to exp
lain the peak sharpening and modified Van Deemter plots found with lar
ge-pore particles. In the second part, column efficiencies in terms of
HETP as a function of bed superficial velocity are experimentally mea
sured for a non-retained protein (bovine serum albumine, BSA) in two a
dsorbents: POROS Q/M (PerSeptive Biosystems) and Q Hyper D (BioSepra).
In the third section breakthrough curves for both materials are measu
red for a test protein (BSA) from which useful capacities and producti
vities as a function of flow-rate are calculated. Experimental results
indicate that intraparticle convection plays indeed an important role
in both adsorbents.