RAPID-DETERMINATION OF THE RATIOS OF 3 AROMATIC RESIDUES IN PEPTIDES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A HIGH-RESOLUTION PHOTODIODE-ARRAY DETECTOR

A method for the simultaneous determination of the ratios of three aro matic residues in peptides by derivative UV spectrophotometry on a spe ctrophotometer with a resolution of 0.1 nm can be used for the RP-HPLC analysis of peptides because of the recent development of high-resolu tion photodiode-array detectors (1.2 nm). The difference between the t heoretical and experimental ratios of aromatic residues of peptides de termined in real time is less than 5%. This method could become a powe rful tool for the study of peptides and hydrolysates. A variety of pos sible applications are discussed.