E. Appel et al., IMPROVED PCR AMPLIFICATION HHAL RESTRICTION FOR UNAMBIGUOUS DETERMINATION OF APOLIPOPROTEIN-E ALLELES, Clinical chemistry, 41(2), 1995, pp. 187-190
We present a modification to the polymerase chain reaction amplificati
on/Hhal restriction isotyping method for human apolipoprotein (ape) E.
This method includes a mutagenic forward primer and 5'-end labeling o
f both primers. These modifications of the original method described b
y Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and un
ambiguous determination of apoE genotypes.