HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF NONAMIDATED ANDGLYCINE-AMIDATED AND TAURINE-AMIDATED BILE-ACID 3-GLUCOSIDES

The high-performance liquid chromatographic separation of Phe 3-glucos ides of nonamidated lithocholic, chenodeoxycholic, ursodeoxycholic, de oxycholic and cholic acids, and their double conjugate forms with glyc ine and taurine has been carried out on a C18 reversed-phase column. S atisfactory separation not only of each the three groups of the nonami dated and amidated bile acid 3-glucosides but also of the individual c ompounds in the same group was attained by employing acetonitrile-0.3% potassium phosphate buffer (pH, 7.0) as mobile phase. The retention d ata reported here provide an insight into structural elucidation of th ese biologically important bile acid 3-glucosides.