SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS BY PERPENDICULAR TEMPERATURE-GRADIENT GEL-ELECTROPHORESIS

Using a newly developed temperature-gradient gel electrophoresis appar atus, mutations in the K-ras oncogene and p53 tumor supressor gene wer e analyzed for single-strand conformation polymorphism (SSCP). The mob ilities of single-stranded DNAs, carrying various mutations, change - depending on the gel temperature during electrophoresis. Therefore, te mperature-gradient single-strand conformation polymorphism (TG-SSCP) a nalysis can provide useful information concerning the optimum temperat ure for SSCP and may also be used to screen for various mutations or p olymorphisms in a single electrophoretic run.