SPECIES IDENTIFICATION OF CYST AND ROOT-KNOT NEMATODES FROM POTATO BYELECTROPHORESIS OF INDIVIDUAL FEMALES

Authors
KARSSEN G VANHOENSELAAR T VERKERKBAKKER B JANSSEN R
Citation
G. Karssen et al., SPECIES IDENTIFICATION OF CYST AND ROOT-KNOT NEMATODES FROM POTATO BYELECTROPHORESIS OF INDIVIDUAL FEMALES, Electrophoresis, 16(1), 1995, pp. 105-109
Citations number
17
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
16
Issue
1
Year of publication
1995
Pages
105 - 109
Database
ISI
SICI code
0173-0835(1995)16:1<105:SIOCAR>2.0.ZU;2-J
Abstract
To carry out rapid and reliable species identification of Globodera an d Meloidogyne specimens, two electrophoretic methods were modified and adapted for an automated electrophoresis system (PhastSystem, Pharmac ia). Proteins of individual Globadera cysts were identified using isoe lectric focusing and a sensitive silver stain. Proteins of young singl e Meloidogyne females were separated using polyacrylamide gradient gel s and stained for the isozymes esterase and malate dehydrogenase.