PREDICTED STRUCTURE OF THE ADENOVIRUS DNA-BINDING PROTEIN

The DNA sequence of a portion of the MAV1 SmaI-D fragment coding for t he C-terminal 147 amino acids of the adenoviral DNA-binding protein (D BP) has been determined. A multiple sequence alignment was constructed of the MAV1 fragment and the DBPs of Ad.2, 4, 5, 7, 12, 40, and 41 to examine the degree of conservation of features that have been mapped on the Ad.2 DBP and to identify further conserved features. The less c onserved N-terminal segment of the protein contains two nuclear locali zation signals and two acidic regions, the host range region, and all of the 11 phosphorylation sites. The highly conserved C-terminal segme nt contains a potential leucine zipper and zinc finger motifs. These s equence features were mapped onto a predicted secondary structure of t he Ad.2 DBP.