We describe and validate a new minimally invasive method for continuou
s measurement of tracheal diameter in anesthetized dogs. The method is
based on measuring displacement of water into and out of a modified e
ndotracheal tube cuff placed in the trachea. The system was calibrated
to allow tracheal diameter to be calculated from known cuff volume. T
he resolution of the method in measuring changes in tracheal diameter
is 0.1 mm over a range of similar to 10-25 mm. The apparatus was teste
d in five dogs by observing the response of the trachea to four stimul
i previously shown to alter tracheal tone: stimulation of nasal mucosa
, hyperinflation of the lungs, induction of hypocapnea, and infusion o
f atropine. The observed changes in tracheal diameter were generally c
onsistent with those of previous studies. The direction and extent of
changes in tracheal diameter in response to the test conditions were c
onfirmed by fluoroscopy. We conclude that continuous measurement of vo
lume changes in the cuff reflects corresponding changes in tracheal di
ameter.